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Abstract of Poster Presentation

Analysis of Cercospora beticola mating type gene structure in north central USA
M. BOLTON (1), G. Secor (2), L. Campbell (3), V. Rivera (2), J. Rengifo (2), J. Weiland (3)
(1) USDA ARS, Fargo, ND, USA; (2) North Dakota State University; (3) USDA ARS
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Cercospora beticola Sacc. is a necrotrophic fungus that causes Cercospora leaf spot of sugarbeet (Beta vulgaris). The fungus is considered to be the most destructive foliar pathogen of sugarbeet worldwide, causing significant economic losses primarily due to reduction in sucrose concentration and root yield. Field isolates of C. beticola are well-known for high levels of variability, particularly with regard to morphological characteristics and fungicide resistance. Nonetheless, despite the diversity found in C. beticola populations, no teleomorph has been described for C. beticola. The mating type genes MAT1-1-1 and MAT1-2-1 genes of C. beticola were recently cloned and used to characterize populations in Western Europe, Iran, and New Zealand, but no such work has been carried out on North America isolates of C. beticola to our knowledge. We therefore characterized the mating type gene structure of C. beticola isolates from sugarbeet production area of north central United States. In addition, isolates were tested for sensitivity to several classes of fungicide to determine if mating type correlated with variability in fungicide sensitivity. A detailed analysis of mating type gene structure and the relationship to fungicide sensitivity will be presented.

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